SSC Buffer 20X (Biotechnology Grade)

$95.00

Catalog Number: B2010086 (250 mL)
SSC Buffer (20X) is a highly reproducible buffer for nucleic acid hybridization techniques. Working concentrations can be prepared by diluting the provided concentrate with di-water to a final concentration of 0.1X to 20X (depending on the application). Custom bulk orders are available upon request.

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Description

SSC Buffer 20X (Biotechnology Grade)

Catalog number: B2010086
Lot number: Batch Dependent
Expiration date: Batch Dependent
Volume/Weight 250 mL
pH 7
Supplied as 20X concentrate (working concentration ranges between 0.1X and 20X depending on application)
Appearance Clear solution
Applications SSC Buffer (20X) is a highly reproducible buffer for nucleic acid hybridization techniques. Working concentrations can be prepared by diluting the provided concentrate with di-water to a final concentration of 0.1X to 20X (depending on the application).
Shelf-Life 1 year from date of manufacture
Storage: Keep at 2-8°C
Keywords: SSC buffer, SSC buffer 20X concentrate, ssc buffer in situ hybridization, saline-sodium citrate buffer, saline-sodium citrate 20X, ssc buffer dna hybridization
Grade Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.
References 1: Goldau T, Murayama K, Brieke C, Asanuma H, Heckel A. Azobenzene C-Nucleosides
for Photocontrolled Hybridization of DNA at Room Temperature. Chemistry. 2015
Dec 1;21(49):17870-6.2: Markegard CB, Gallivan CP, Cheng DD, Nguyen HD. Effects of Concentration and
Temperature on DNA Hybridization by Two Closely Related Sequences via Large-
Scale Coarse-Grained Simulations. J Phys Chem B. 2016 Aug 18;120(32):7795-806.

3: do Nascimento C, Muller K, Sato S, Albuquerque Junior RF. Effect of sample
storage time on detection of hybridization signals in Checkerboard DNA-DNA
hybridization. Can J Microbiol. 2012 Apr;58(4):502-6.
4: Avarre JC, de Lajudie P, Béna G. Hybridization of genomic DNA to microarrays:
a challenge for the analysis of environmental samples. J Microbiol Methods. 2007
May;69(2):242-8.
5: Cho JC, Tiedje JM. Bacterial species determination from DNA-DNA hybridization
by using genome fragments and DNA microarrays. Appl Environ Microbiol. 2001
Aug;67(8):3677-82.
6: Su X, Wu YJ, Knoll W. Comparison of surface plasmon resonance spectroscopy
and quartz crystal microbalance techniques for studying DNA assembly and
hybridization. Biosens Bioelectron. 2005 Nov 15;21(5):719-26.

7: Hu Y, Yang T, Li Q, Guan Q, Jiao K. Conjugated self-doped polyaniline-DNA
hybrid as trigger for highly sensitive reagentless and electrochemical self-
signal amplifying DNA hybridization sensing. Analyst. 2013 Feb
21;138(4):1067-74.

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