RNA and DNA Endonuclease from Serratia marcescens

Product Description

RNA and DNA Endonuclease from Serratia marcescens
Catalog number: B2014452
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 1 kU
Molecular Weight or Concentration: N/A
Supplied as: Solution
Applications: molecular tool for various biochemical applications
Storage: −20°C
Keywords: Endonucleaase
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Menacho-Melgar R, Moreb EA, Efromson JP, Yang T, Hennigan JN, Wang R, Lynch MD. Improved two-stage protein expression and purification via autoinduction of both autolysis and auto DNA/RNA hydrolysis conferred by phage lysozyme and DNA/RNA endonuclease Biotechnol Bioeng. 2020 Sep;117(9):2852-2860.
2: Ahrenholtz I, Lorenz MG, Wackernagel W. The extracellular nuclease of Serratia marcescens: studies on the activity in vitro and effect on transforming DNA in a groundwater aquifer microcosm Arch Microbiol. 1994;161(2):176-83.
3: Franke I, Meiss G, Blecher D, Gimadutdinow O, Urbanke C, Pingoud A. Genetic engineering, production and characterisation of monomeric variants of the dimeric Serratia marcescens endonuclease FEBS Lett. 1998 Apr 3;425(3):517-22.
4: Miller MD, Benedik MJ, Sullivan MC, Shipley NS, Krause KL. Crystallization and preliminary crystallographic analysis of a novel nuclease from Serratia marcescens J Mol Biol. 1991 Nov 5;222(1):27-30.
5: Meiss G, Gast FU, Pingoud AM. The DNA/RNA non-specific Serratia nuclease prefers double-stranded A-form nucleic acids as substrates J Mol Biol. 1999 May 7;288(3):377-90.
6: Miller MD, Krause KL. Identification of the Serratia endonuclease dimer: structural basis and implications for catalysis Protein Sci. 1996 Jan;5(1):24-33.
7: Miller MD, Tanner J, Alpaugh M, Benedik MJ, Krause KL. 2.1 A structure of Serratia endonuclease suggests a mechanism for binding to double-stranded DNA Nat Struct Biol. 1994 Jul;1(7):461-8.
8: Pommer AJ, Cal S, Keeble AH, Walker D, Evans SJ, Kühlmann UC, Cooper A, Connolly BA, Hemmings AM, Moore GR, James R, Kleanthous C. Mechanism and cleavage specificity of the H-N-H endonuclease colicin E9 J Mol Biol. 2001 Dec 7;314(4):735-49.
9: Friedhoff P, Meiss G, Kolmes B, Pieper U, Gimadutdinow O, Urbanke C, Pingoud A. Kinetic analysis of the cleavage of natural and synthetic substrates by the Serratia nuclease Eur J Biochem. 1996 Oct 15;241(2):572-80.
10: Lu JJ, Perng CL, Lee SY, Wan CC. Use of PCR with universal primers and restriction endonuclease digestions for detection and identification of common bacterial pathogens in cerebrospinal fluid J Clin Microbiol. 2000 Jun;38(6):2076-80.

Products Related to RNA and DNA Endonuclease from Serratia marcescens can be found at Enzymes