Protein A–Sepharose

Product Description

Protein A–Sepharose
Catalog number: B2014680
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 1 mL
Molecular Weight or Concentration: N/A
Supplied as: Solution
Applications: molecular tool for various biochemical applications
Storage: 2-8°C
Keywords: Protein A–Agarose macrobeads
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Yaciuk P. Co-immunoprecipitation of protein complexes Methods Mol Med. 2007;131:103-11.
2: Paggi RA, De Castro RE, Cerletti M. In Vivo Protein Cross-Linking and Coimmunoprecipitation in Haloferax volcanii Methods Mol Biol. 2022;2522:301-317.
3: van der Geer P. Analysis of protein-protein interactions by coimmunoprecipitation Methods Enzymol. 2014;541:35-47.
4: Dickson C. Protein techniques: immunoprecipitation, in vitro kinase assays, and Western blotting Methods Mol Biol. 2008;461:735-44.
5: Gao J, Tian H, Wang Y, Yang Q, Liu D, Wang Y, Mi H. The design of protein-imprinted polymers as antibody substitutes for investigating protein-protein interactions Biomaterials. 2012 Apr;33(11):3344-52.
6: Kent UM. Purification of antibodies using protein A-sepharose and FPLC Methods Mol Biol. 1999;115:29-33.
7: O’Neill LP, Turner BM. Immunoprecipitation of chromatin Methods Enzymol. 1996;274:189-97.
8: Young WW Jr, Tamura Y, Wolock DM, Fox JW. Staphylococcal protein A binding to the Fab fragments of mouse monoclonal antibodies J Immunol. 1984 Dec;133(6):3163-6.
9: Peng Z, Becker AB, Simons FE. Binding properties of protein A and protein G for human IgE Int Arch Allergy Immunol. 1994;104(2):204-6.
10: Beaudreuil S, Zhang X, Kriaa F, Dantal J, Francois H, Vazquez A, Charpentier B, Lorenzo HK, Durrbach A. Protein A immunoadsorption cannot significantly remove the soluble receptor of urokinase from sera of patients with recurrent focal segmental glomerulosclerosis Nephrol Dial Transplant. 2014 Feb;29(2):458-63.

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