Luciferase Substrate

Description

Luciferase Substrate
Catalog number: B2014209
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 5 mg
Molecular Weight or Concentration: 305.38 g/mol
Supplied as: Powder
Applications: molecular tool for various biochemical applications
Storage: −20°C
Keywords: Luciferase Substrate, CycLuc1
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Kim M, Gupta SK, Zhang W, Talele S, Mohammad AS, Laramy J, Mladek AC, Zhang S, Sarkaria JN, Elmquist WF. Factors Influencing Luciferase-Based Bioluminescent Imaging in Preclinical Models of Brain Tumor Drug Metab Dispos. 2022 Mar;50(3):277-286.
2: Hendrickx S, Bult D, Mabille D, Mols R, Claes M, Ilbeigi K, Ahmad R, Dirkx L, Van Acker SI, Caljon G. Comparison of Bioluminescent Substrates in Natural Infection Models of Neglected Parasitic Diseases Int J Mol Sci. 2022 Dec 16;23(24):16074.
3: Adams ST Jr, Mofford DM, Reddy GS, Miller SC. Firefly Luciferase Mutants Allow Substrate-Selective Bioluminescence Imaging in the Mouse Brain Angew Chem Int Ed Engl. 2016 Apr 11;55(16):4943-6.
4: Simonyan H, Hurr C, Young CN. A synthetic luciferin improves in vivo bioluminescence imaging of gene expression in cardiovascular brain regions Physiol Genomics. 2016 Oct 1;48(10):762-770.
5: Zambito G, Gaspar N, Ridwan Y, Hall MP, Shi C, Kirkland TA, Encell LP, Lwik C, Mezzanotte L. Evaluating Brightness and Spectral Properties of Click Beetle and Firefly Luciferases Using Luciferin Analogues: Identification of Preferred Pairings of Luciferase and Substrate for In Vivo Bioluminescence Imaging Mol Imaging Biol. 2020 Dec;22(6):1523-1531.
6: Kleinovink JW, Mezzanotte L, Zambito G, Fransen MF, Cruz LJ, Verbeek JS, Chan A, Ossendorp F, Lwik C. A Dual-Color Bioluminescence Reporter Mouse for Simultaneous in vivo Imaging of T Cell Localization and Function Front Immunol. 2019 Jan 8;9:3097.
7: Martin-Burgos B, Wang W, William I, Tir S, Mohammad I, Javed R, Smith S, Cui Y, Arzavala J, Mora D, Smith CB, van der Vinne V, Molyneux PC, Miller SC, Weaver DR, Leise TL, Harrington ME. Methods for Detecting PER2:LUCIFERASE Bioluminescence Rhythms in Freely Moving Mice J Biol Rhythms. 2022 Feb;37(1):78-93.
8: Mezzanotte L, Iljas JD, Que I, Chan A, Kaijzel E, Hoeben R, Lwik C. Optimized Longitudinal Monitoring of Stem Cell Grafts in Mouse Brain Using a Novel Bioluminescent/Near Infrared Fluorescent Fusion Reporter Cell Transplant. 2017 Dec;26(12):1878-1889.
9: Evans MS, Chaurette JP, Adams ST Jr, Reddy GR, Paley MA, Aronin N, Prescher JA, Miller SC. A synthetic luciferin improves bioluminescence imaging in live mice Nat Methods. 2014 Apr;11(4):393-5.
10: Ji X, Adams ST Jr, Miller SC. Bioluminescence imaging in mice with synthetic luciferin analogues Methods Enzymol. 2020;640:165-183.

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