Gel Shift Binding Buffer 5X Solution


Catalog Number: B2010103 (4 x 500 uL)
Gel Shift Binding Buffer 5X is a highly optimized, reproducible, buffer for use in gel electrophoresis mobility shift assay (EMSA) and similar applications. Custom bulk orders are available upon request.

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Gel Shift Binding Buffer 5X

Catalog number: B2010103
Lot number: Batch Dependent
Expiration date: Batch Dependent
Volume/Weight 4 x 500 uL
pH 7.5
Supplied as 5X concentrate
Appearance Clear solution
Applications Binding buffer for gel electrophoresis mobility shift assay (EMSA)
Shelf-Life 1 year from date of manufacture
Storage: Keep at -20°C
Keywords: EMSA Binding buffer, electrophoresis mobility shift assay binding buffer
Grade Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.
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electrophoresis. Curr Protoc Pharmacol. 2001 Aug;Chapter 6:Unit6.8.

6: Künne T, Westra ER, Brouns SJ. Electrophoretic Mobility Shift Assay of DNA
and CRISPR-Cas Ribonucleoprotein Complexes. Methods Mol Biol. 2015;1311:171-84.

7: Csorba T, Burgyán J. Gel mobility shift assays for RNA binding viral RNAi
suppressors. Methods Mol Biol. 2011;721:245-52.

8: Poulin-Laprade D, Burrus V. Electrophoretic Mobility Shift Assay Using
Radiolabeled DNA Probes. Methods Mol Biol. 2015;1334:1-15.

9: Sadakane Y, Hatanaka Y. Photoaffinity electrophoretic mobility shift assay
using photoreactive DNA bearing 3-trifluoromethyl-3-phenyldiazirine in its
phosphate backbone. Anal Biochem. 2016 Aug 1;506:1-7.

10: Carruthers NJ, Parker GC, Gratsch T, Caruso JA, Stemmer PM. Protein Mobility
Shifts Contribute to Gel Electrophoresis Liquid Chromatography Analysis. Version
2. J Biomol Tech. 2015 Sep;26(3):103-12.

11: Memelink J. Electrophoretic mobility shift assay for the analysis of
interactions of jasmonic acid-responsive transcription factors with DNA. Methods
Mol Biol. 2013;1011:209-25.

12: García-Solaesa V, Sanz-Lozano CS. Interactions of DNA and Proteins:
Electrophoretic Mobility Shift Assay in Asthma. Methods Mol Biol.

13: Morita T, Maki K, Aiba H. Detection of sRNA-mRNA interactions by
electrophoretic mobility shift assay. Methods Mol Biol. 2012;905:235-44. d

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