Enteropeptidase Fluorogenic Substrate

Product Description

Enteropeptidase Fluorogenic Substrate
Catalog number: B2012917
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 500 ug
Molecular Weight or Concentration: 859.7 g/mol
Supplied as: Lyophilized Powder
Applications: molecular tool for various biochemical applications
Storage: -20°C
Keywords: (3S,6S,9S,12S,15S)-3-acetamido-19-amino-6,9,12-tris(carboxymethyl)-4,7,10,13-tetraoxo-15-((2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl)carbamoyl)-5,8,11,14-tetraazanonadecanoic acid, trifluoroacetate salt
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Yang X, Ding Z, Peng L, Song Q, Zhang D, Cui F, Xia C, Li K, Yin H, Li S, Li Z, Huang H. Cryo-EM structures reveal the activation and substrate recognition mechanism of human enteropeptidase Nat Commun. 2022 Nov 14;13(1):6955.
2: Zheng XL, Kitamoto Y, Sadler JE. Enteropeptidase, a type II transmembrane serine protease Front Biosci (Elite Ed). 2009 Jun 1;1(1):242-9.
3: Antonowicz I, Hesford FJ, Green JR, Grogg P, Hadorn B. The application of a new synthetic substrate to the determination of enteropeptidase in rat small intestine and human intestinal biopsies Clin Chim Acta. 1980 Feb 14;101(1):69-76.
4: Azhar M, Somashekhar R. Production and purification of recombinant enteropeptidase expressed in an insect-baculovirus cell system Prep Biochem Biotechnol. 2015;45(3):268-78.
5: Hesford F, Hadorn B, Blaser K, Schneider CH. A new substrate for enteropeptidase FEBS Lett. 1976 Dec 1;71(2):279-82.
6: Choi MG, Lee E, Chung HS, Jang SH, Lee C. A fluorogenic method for measuring enteropeptidase activity: spectral shift in the emission of GD4K-conjugated 7-amino-4-methylcoumarin BMB Rep. 2011 Jul;44(7):458-61.
7: Yang X, Yin H, Peng L, Zhang D, Li K, Cui F, Xia C, Huang H, Li Z. The Global Status and Trends of Enteropeptidase: A Bibliometric Study Front Med (Lausanne). 2022 Feb 10;9:779722.
8: Smith ET, Johnson DA. Human enteropeptidase light chain: bioengineering of recombinants and kinetic investigations of structure and function Protein Sci. 2013 May;22(5):577-85.
9: Ostapchenko VG, Gasparian ME, Kosinsky YA, Efremov RG, Dolgikh DA, Kirpichnikov MP. Dissecting structural basis of the unique substrate selectivity of human enteropeptidase catalytic subunit J Biomol Struct Dyn. 2012;30(1):62-73.
10: Jenö P, Green JR, Lentze MJ. Specificity studies on enteropeptidase substrates related to the N-terminus of trypsinogen Biochem J. 1987 Feb 1;241(3):721-7.

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