Dihydroethidium (Powder)

Description

Dihydroethidium (Powder)
Catalog number: B2011426
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 5 mg
Molecular Weight or Concentration: 315.4 g/mol
Supplied as: Lyophilized
Applications: molecular tool for various biochemical applications
Storage: -20 °C
Keywords: Hydroethidine
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Yu J, Wang WN, Matei N, Li X, Pang JW, Mo J, Chen SP, Tang JP, Yan M, Zhang JH. Ezetimibe Attenuates Oxidative Stress and Neuroinflammation via the AMPK/Nrf2/TXNIP Pathway after MCAO in Rats Oxid Med Cell Longev. 2020 Jan 4;2020:4717258.
2: Hu C, Zhang X, Wei W, Zhang N, Wu H, Ma Z, Li L, Deng W, Tang Q. Matrine attenuates oxidative stress and cardiomyocyte apoptosis in doxorubicin-induced cardiotoxicity via maintaining AMPKα/UCP2 pathway Acta Pharm Sin B. 2019 Jul;9(4):690-701.
3: Laurindo FR, Fernandes DC, Santos CX. Assessment of superoxide production and NADPH oxidase activity by HPLC analysis of dihydroethidium oxidation products Methods Enzymol. 2008;441:237-60.
4: Wang Q, Zou MH. Measurement of Reactive Oxygen Species (ROS) and Mitochondrial ROS in AMPK Knockout Mice Blood Vessels Methods Mol Biol. 2018;1732:507-517.
5: Weng CC, Carlin S, Hou C, Metz T, Li S, Lee H, Xu K, Makvandi M, Mach RH. Correlation analysis of [(18)F]ROStrace using ex vivo autoradiography and dihydroethidium fluorescent imaging in lipopolysaccharide-treated animals Biochem Biophys Res Commun. 2019 Aug 20;516(2):397-401.
6: Wojtala A, Bonora M, Malinska D, Pinton P, Duszynski J, Wieckowski MR. Methods to monitor ROS production by fluorescence microscopy and fluorometry Methods Enzymol. 2014;542:243-62.
7: Johnson-Cadwell LI, Jekabsons MB, Wang A, Polster BM, Nicholls DG. ‘Mild Uncoupling’ does not decrease mitochondrial superoxide levels in cultured cerebellar granule neurons but decreases spare respiratory capacity and increases toxicity to glutamate and oxidative stress J Neurochem. 2007 Jun;101(6):1619-31.
8: Abubaker AA, Vara D, Eggleston I, Canobbio I, Pula G. A novel flow cytometry assay using dihydroethidium as redox-sensitive probe reveals NADPH oxidase-dependent generation of superoxide anion in human platelets exposed to amyloid peptide β Platelets. 2019;30(2):181-189.
9: Ashley N, Harris D, Poulton J. Detection of mitochondrial DNA depletion in living human cells using PicoGreen staining Exp Cell Res. 2005 Feb 15;303(2):432-46.
10: Chung CY, Duchen MR. A Plate Reader-Based Measurement of the Cellular ROS Production Using Dihydroethidium and MitoSOX Methods Mol Biol. 2022;2497:333-337.

Products Related to Dihydroethidium (Powder) can be found at Dyes