Coomassie Brilliant Blue G-250

Product Description

Coomassie Brilliant Blue G-250
Catalog number: B2016520
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 1 g
Molecular Weight or Concentration: 854.02 g/mol
Supplied as: Powder
Applications: a molecular tool for various biochemical applications
Storage: RT
Keywords: CBBG
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Georgiou CD, Grintzalis K, Zervoudakis G, Papapostolou I. Mechanism of Coomassie brilliant blue G-250 binding to proteins: a hydrophobic assay for nanogram quantities of proteins Anal Bioanal Chem. 2008 May;391(1):391-403.
2: Kielkopf CL, Bauer W, Urbatsch IL. Bradford Assay for Determining Protein Concentration Cold Spring Harb Protoc. 2020 Apr 1;2020(4):102269.
3: Dyballa N, Metzger S. Fast and sensitive colloidal coomassie G-250 staining for proteins in polyacrylamide gels J Vis Exp. 2009 Aug 3;(30):1431.
4: Kruger NJ. The Bradford method for protein quantitation Methods Mol Biol. 1994;32:9-15.
5: Ernst O, Zor T. Linearization of the bradford protein assay J Vis Exp. 2010 Apr 12;(38):1918.
6: Simonian MH. Spectrophotometric determination of protein concentration Curr Protoc Cell Biol. 2002 Aug;Appendix 3:Appendix 3B.
7: Kielkopf CL, Bauer W, Urbatsch IL. Methods for Measuring the Concentrations of Proteins Cold Spring Harb Protoc. 2020 Apr 1;2020(4):102277.
8: Pariary R, Dolui S, Shome G, Mohid SA, Saha A, Ratha BN, Harikishore A, Jana K, Mandal AK, Bhunia A, Maiti NC. Coomassie brilliant blue G-250 acts as a potential chemical chaperone to stabilize therapeutic insulin Chem Commun (Camb). 2023 Jun 27;59(52):8095-8098.
9: Zohny MH, Cavalu S, Youssef ME, Kaddah MMY, Mourad AAE, Gaafar AGA, El-Ahwany E, Amin NA, Arakeep HM, Shata A, Saleh S, Hafez MM, Elazab ST, Abdelhady R, El Shahat RM, Yahya G, Saber S. Coomassie brilliant blue G-250 dye attenuates bleomycin-induced lung fibrosis by regulating the NF-κB and NLRP3 crosstalk: A novel approach for filling an unmet medical need Biomed Pharmacother. 2022 Apr;148:112723.
10: Arndt C, Koristka S, Feldmann A, Bergmann R, Bachmann M. Coomassie Brilliant Blue Staining of Polyacrylamide Gels Methods Mol Biol. 2018;1853:27-30.

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