Coomassie Blue R-250 (Highly Pure)

Product Description

Coomassie Blue R-250 (Highly Pure)
Catalog number: B2012364
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 5 g
Molecular Weight or Concentration: 826.0 g/mol
Supplied as: Powder
Applications: molecular tool for various biochemical applications
Storage: -20°C
Keywords: Coomassie blue R-250
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Kumar G. Fungal Laccase Efficiently Destains Coomassie Brilliant Blue-R-250 Stained Polyacrylamide Gels Methods Mol Biol. 2018;1853:247-253.
2: Nagata K, Ashikaga R, Mori W, Zako T, Shimazaki Y. Analysis of the enzymatic degradation of lysozyme fibrils using a combination method of non-denaturing gel electrophoresis and double staining with Coomassie Brilliant Blue G-250 and R-250 dyes Anal Sci. 2022 Nov 30.
3: Yang J, Yang X, Ye X, Lin J. Destaining of Coomassie Brilliant Blue R_250-stained polyacrylamide gels with fungal laccase Anal Biochem. 2016 Jan 15;493:27-9.
4: Yang J, Yang X, Ye X, Lin J. Optimal parameters for laccase-mediated destaining of Coomassie Brilliant Blue R_250-stained polyacrylamide gels Data Brief. 2016 Jan 29;7:1-7.
5: Goldring JPD. The Roles of Acetic Acid and Methanol During Fixing and Staining Proteins in an SDS-Polyacrylamide Electrophoresis Gel Methods Mol Biol. 2018;1853:15-18.
6: Hu PP, Liu H, Zhan L, Zheng LL, Huang CZ. Coomassie brilliant blue R_250 as a new surface-enhanced Raman scattering probe for prion protein through a dual-aptamer mechanism Talanta. 2015 Jul 1;139:35-9.
7: Hattori S, Sakai K, Watanabe K, Fujii T. The induction of metachromasia and circular dichroism of coomassie brilliant blue R_250 with collagen and histone H1 is due to the low content of hydrophobic amino acid residues in these proteins J Biochem. 1996 Mar;119(3):400-6.
8: Rosenthal HL, Berger RA, Tyler AN, Moore BW. Isolation of a component from commercial coomassie brilliant blue R_250 that stains rubrophilin and other proteins red on polyacrylamide gels Biochim Biophys Acta. 1988 May 12;965(2-3):106-13.
9: Duhamel RC, Meezan E, Brendel K. Metachromatic staining with Coomassie Brilliant Blue R_250 of the proline-rich calf thymus histone, H1 Biochim Biophys Acta. 1980 Dec 16;626(2):432-42.
10: Sreeramulu G, Singh NK. Destaining of Coomassie Brilliant Blue R-250-stained polyacrylamide gels with sodium chloride solutions Electrophoresis. 1995 Mar;16(3):362-5.

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