5,5′-Dithiobis(2-nitrobenzoic Acid) DTNB

Product Description

5,5′-Dithiobis(2-nitrobenzoic Acid) DTNB
Catalog number: B2010677
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 1 g
Molecular Weight or Concentration: 396.35 g/mol
Supplied as: Powder
Applications: molecular tool for the study of thiol-containing molecules
Storage: RT
Keywords: DTNB, Bis(3-carboxy-4-nitrophenyl) disulfide, Ellman’s Reagent, 3-Carboxy-4-nitrophenyl disulfide, 6,6′-Dinitro-3,3′-dithiodibenzoic acid
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Owen JB, Butterfield DA. Measurement of oxidized/reduced glutathione ratio Methods Mol Biol. 2010;648:269-77.
2: Sullivan ML, Bonawitz ND. Spectrophotometric determination of reaction rates and kinetic parameters of a BAHD acyltransferase using DTNB (5,5′-dithio-bis-[2-nitrobenzoic acid]) Plant Sci. 2018 Apr;269:148-152.
3: Xing B, Shi Y, Tang W. Kinetic studies of the reactions of some metal reconstituted metallothioneins with the electrophilic disulfide 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB) Biometals. 2000 Dec;13(4):295-300.
4: Lara HH, Ixtepan-Turrent L, Garza-Treviño EN, Flores-Teviño SM, Borkow G, Rodriguez-Padilla C. Antiviral propierties of 5,5′-dithiobis-2-nitrobenzoic acid and bacitracin against T-tropic human immunodeficiency virus type 1 Virol J. 2011 Mar 24;8:137.
5: Huang BH, Shen SS, Wei N, Guo XF, Wang H. Fluorescence biosensor based on silicon quantum dots and 5,5′-dithiobis-(2-nitrobenzoic acid) for thiols in living cells Spectrochim Acta A Mol Biomol Spectrosc. 2020 Mar 15;229:117972.
6: Takeda K, Shigemura A, Hamada S, Gu W, Fang D, Sasa K, Hachiya K. Dependence of reaction rate of 5,5′-dithiobis-(2-nitrobenzoic acid) to free sulfhydryl groups of bovine serum albumin and ovalbumin on the protein conformations J Protein Chem. 1992 Apr;11(2):187-92.
7: Reithmeier RA. Inhibition of anion transport in human red blood cells by 5,5′-dithiobis(2-nitrobenzoic acid) Biochim Biophys Acta. 1983 Jul 13;732(1):122-5.
8: Li ZG. Quantification of hydrogen sulfide concentration using methylene blue and 5,5′-dithiobis(2-nitrobenzoic acid) methods in plants Methods Enzymol. 2015;554:101-10.
9: Chen W, Zhao Y, Seefeldt T, Guan X. Determination of thiols and disulfides via HPLC quantification of 5-thio-2-nitrobenzoic acid J Pharm Biomed Anal. 2008 Dec 15;48(5):1375-80.
10: Ghiggeri GM, Candiano G, Delfino G, Queirolo C. A modification of the 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB) method for the determination of the sulphhydryl content of human serum albumin Clin Chim Acta. 1983 May 30;130(2):257-61.

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