5-Bromo-4-chloro-3-indolyl β-D-Galactopyranoside


Catalog Number: B2012172 (50 mg)
5-Bromo-4-chloro-3-indolyl β-D-Galactopyranoside is a high quality 5-Bromo-4-chloro-3-indolyl β-D-Galactopyranoside (>98.0% by HPLC). This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

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5-Bromo-4-chloro-3-indolyl β-D-Galactopyranoside
Catalog number: B2012172
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 50 mg
Molecular Weight or Concentration: 408.63 g/mol
Supplied as: Powder
Applications: molecular tool for various biochemical applications
Storage: -20°C
Keywords: 5-Bromo-4-chloro-3-(β-D-galactopyranosyloxy)indole, X-Gal
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

1: Itahana K, Campisi J, Dimri GP. Methods to detect biomarkers of cellular senescence: the senescence-associated beta-galactosidase assay Methods Mol Biol. 2007;371:21-31.
2: Gary RK, Kindell SM. Quantitative assay of senescence-associated beta-galactosidase activity in mammalian cell extracts Anal Biochem. 2005 Aug 15;343(2):329-34.
3: Aspelund A, Antila S, Proulx ST, Karlsen TV, Karaman S, Detmar M, Wiig H, Alitalo K. A dural lymphatic vascular system that drains brain interstitial fluid and macromolecules J Exp Med. 2015 Jun 29;212(7):991-9.
4: Green MR, Sambrook J. Screening Bacterial Colonies Using X-Gal and IPTG: α-Complementation Cold Spring Harb Protoc. 2019 Dec 2;2019(12).
5: Lozano-Gerona J, García-Otín ÁL. ImageJ-based semiautomatic method to analyze senescence in cell culture Anal Biochem. 2018 Feb 15;543:30-32.
6: Rodig SJ. Detecting β-Galactosidase-Labeled Cells Cold Spring Harb Protoc. 2020 May 1;2020(5):099739.
7: Arakawa H, Ikegami T, Maeda M, Tsuji A. Chemiluminescent enzyme immunoassay for alpha-fetoprotein using beta-D-galactosidase as label and 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside as substrate J Biolumin Chemilumin. 1993 May-Jun;8(3):135-9.
8: Ley A, Barr S, Fredenburgh D, Taylor M, Walker N. Use of 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside for the isolation of beta-galactosidase-positive bacteria from municipal water supplies Can J Microbiol. 1993 Sep;39(9):821-5.
9: Kobayashi S, Okada M. A double staining technique using 5-bromo,4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal) and immunoperoxidase in whole Drosophila embryos Biotech Histochem. 1993 Jul;68(4):237-9.
10: Krämer MS, Feil R, Schmidt H. Analysis of Gene Expression Using lacZ Reporter Mouse Lines Methods Mol Biol. 2021;2224:29-45.

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