4-Nitrophenyl β-D-Xylopyranoside


Catalog Number: B2012246 (50 mg)
4-Nitrophenyl β-D-Xylopyranoside is a high quality Substrate for β-Xylosidase. This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

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4-Nitrophenyl β-D-Xylopyranoside
Catalog number: B2012246
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 50 mg
Molecular Weight or Concentration: 271.2 g/mol
Supplied as: Powder
Applications: molecular tool for various biochemical applications
Storage: -20C
Keywords: PNPX
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

1: Mastihubová M, Biely P. Lipase-catalysed preparation of acetates of 4-nitrophenyl beta-D-xylopyranoside and their use in kinetic studies of acetyl migration Carbohydr Res. 2004 May 17;339(7):1353-60.
2: Wong YC, Chan L. Effects of p-nitrophenyl-beta-D-xylopyranoside (beta-D-xyloside) on the androgen-induced growth of the lateral prostate of the prepubertally castrated guinea pig Prostate. 1993;23(1):37-59.
3: Puchart V, Biely P. A simple enzymatic synthesis of 4-nitrophenyl beta-1,4-D-xylobioside, a chromogenic substrate for assay and differentiation of endoxylanases J Biotechnol. 2007 Feb 20;128(3):576-86.
4: Takeo K, Ohguchi Y, Hasegawa R, Kitamura S. Synthesis of 2- and 4-nitrophenyl beta-glycosides of beta-(1–>4)- D-xylo-oligosaccharides of dp 2-4 Carbohydr Res. 1995 Nov 22;277(2):231-44.
5: Biely P, Mastihubová M, Tenkanen M, Eyzaguirre J, Li XL, Vršanská M. Action of xylan deacetylating enzymes on monoacetyl derivatives of 4-nitrophenyl glycosides of β-D-xylopyranose and α-L-arabinofuranose J Biotechnol. 2011 Jan 10;151(1):137-42.
6: Fogelfeld L, Schneider AB. Inhibition of chondroitin sulfate incorporation into human thyroglobulin by p-nitrophenyl-beta-D-xylopyranoside Endocrinology. 1990 Feb;126(2):1064-9.
7: Haggerty JG, Bretton RH, Milstone LM. Response of stratified cultures of human keratinocytes to disruption of proteoglycan synthesis by p-nitrophenyl-beta-D-xylopyranoside J Cell Physiol. 1994 Jan;158(1):39-46.
8: Biely P, Mastihubová M, la Grange DC, van Zyl WH, Prior BA. Enzyme-coupled assay of acetylxylan esterases on monoacetylated 4-nitrophenyl beta-D-xylopyranosides Anal Biochem. 2004 Sep 1;332(1):109-15.
9: Rather MY, Mishra S, Aravinda S. Exploring the synthetic potential of cell bound β-glycosidase of Pichia etchellsii J Biotechnol. 2013 May 10;165(1):63-8.
10: Eneyskaya EV, Ivanen DR, Bobrov KS, Isaeva-Ivanova LS, Shabalin KA, Savel’ev AN, Golubev AM, Kulminskaya AA. Biochemical and kinetic analysis of the GH3 family beta-xylosidase from Aspergillus awamori X-100 Arch Biochem Biophys. 2007 Jan 15;457(2):225-34.

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