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4-Methylumbelliferyl-β D-cellobioside

Original price was: $1,395.00.Current price is: $695.00.

Catalog Number: B2015016 (25 mg)
4-Methylumbelliferyl-β D-cellobioside is a high quality fluorogenic substrate used to measure the activity of (1,4)-β-Glucanase. This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

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Product Description

4-Methylumbelliferyl-β D-cellobioside
Catalog number: B2015016
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 25 mg
Molecular Weight or Concentration: 500.46 g/mol
Supplied as: Powder
Applications: molecular tool for various biochemical applications
Storage: -20° C
Keywords: 4-Methylumbelliferyl-β-, D-cellobioside, 4-MU-β-D-cellobioside
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Körfer G, Besirlioglu V, Davari MD, Martinez R, Vojcic L, Schwaneberg U. Combinatorial InVitroFlow-assisted mutagenesis (CombIMut) yields a 41-fold improved CelA2 cellulase Biotechnol Bioeng. 2022 Aug;119(8):2076-2087.
2: Woon JS, Mackeen MM, Illias RM, Mahadi NM, Broughton WJ, Murad AMA, Abu Bakar FD. Cellobiohydrolase B of Aspergillus niger over-expressed in Pichia pastoris stimulates hydrolysis of oil palm empty fruit bunches PeerJ. 2017 Oct 12;5:e3909.
3: Wolfgang DE, Wilson DB. Mechanistic studies of active site mutants of Thermomonospora fusca endocellulase E2 Biochemistry. 1999 Jul 27;38(30):9746-51.
4: Ko KC, Han Y, Cheong DE, Choi JH, Song JJ. Strategy for screening metagenomic resources for exocellulase activity using a robotic, high-throughput screening system J Microbiol Methods. 2013 Sep;94(3):311-6.
5: Pedraza-Reyes M, Gutiérrez-Corona F. The bifunctional enzyme chitosanase-cellulase produced by the gram-negative microorganism Myxobacter sp. AL-1 is highly similar to Bacillus subtilis endoglucanases Arch Microbiol. 1997 Oct;168(4):321-7.
6: Yoneda Y, Kawada T, Rosenau T, Kosma P. Synthesis of methyl 4′-O-methyl-13C12-beta-D-cellobioside from 13C6-D-glucose. Part 1: reaction optimization and synthesis Carbohydr Res. 2005 Oct 31;340(15):2428-35.
7: Malz F, Yoneda Y, Kawada T, Mereiter K, Kosma P, Rosenau T, Jäger C. Synthesis of methyl 4′-O-methyl-beta-D-cellobioside-13C12 from D-glucose-13C6. Part 2: solid-state NMR studies Carbohydr Res. 2007 Jan 15;342(1):65-70.
8: Barr BK, Holewinski RJ. 4-Methyl-7-thioumbelliferyl-beta-D-cellobioside: a fluorescent, nonhydrolyzable substrate analogue for cellulases Biochemistry. 2002 Apr 2;41(13):4447-52.
9: Chernoglazov VM, Jafarova AN, Klyosov AA. Continuous photometric determination of endo-1,4-beta-D-glucanase (cellulase) activity using 4-methylumbelliferyl-beta-D-cellobioside as a substrate Anal Biochem. 1989 May 15;179(1):186-9.
10: Meiland M, Heinze T, Guenther W, Liebert T. Studies on the boronation of methyl-beta-D-cellobioside–a cellulose model Carbohydr Res. 2010 Jan 26;345(2):257-63.

Products Related to 4-Methylumbelliferyl-β D-cellobioside can be found at Substrates

Additional Information

Weight 48 oz
Dimensions 8 × 8 × 8 in

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