Neutral Protease (Dispase)

Product Description

Neutral Protease (Dispase)
Catalog number: B2018270
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 5 mg
Molecular Weight or Concentration: 36 kDa
Supplied as: Powder
Applications: a molecular tool for various biochemical applications
Storage: 2-8°C
Keywords: Dispase
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Stenn KS, Link R, Moellmann G, Madri J, Kuklinska E. Dispase, a neutral protease from Bacillus polymyxa, is a powerful fibronectinase and type IV collagenase J Invest Dermatol. 1989 Aug;93(2):287-90.
2: Nagata M, Matsumura T. Action of the bacterial neutral protease, dispase, on cultured cells and its application to fluid suspension culture with a review on biomedical application of this protease Jpn J Exp Med. 1986 Dec;56(6):297-307.
3: Umbach JA, Gundersen CB, Baker PF. Giant synaptosomes Nature. 1984 Oct 4-10;311(5985):474-7.
4: O’Gorman D, Kin T, Pawlick R, Imes S, Senior PA, Shapiro AM. Clinical islet isolation outcomes with a highly purified neutral protease for pancreas dissociation Islets. 2013 May-Jun;5(3):111-5.
5: Craviso GL. Generation of functionally competent single bovine adrenal chromaffin cells from cell aggregates using the neutral protease dispase J Neurosci Methods. 2004 Aug 30;137(2):275-81.
6: Fiebig D, Storka J, Roeder M, Meyners C, Schmelz S, Blankenfeldt W, Scrima A, Kolmar H, Fuchsbauer HL. Destructive twisting of neutral metalloproteases: the catalysis mechanism of the Dispase autolysis-inducing protein from Streptomyces mobaraensis DSM 40487 FEBS J. 2018 Nov;285(22):4246-4264.
7: Fujita T, Shiota K, Yoshikawa J, Ogawa S, Aoyagi H. Simple method for analyzing the purity of protease-containing samples by acid-treatment SDS-PAGE J Biosci Bioeng. 2019 Nov;128(5):630-635.
8: Kitano Y, Okada N. Separation of the epidermal sheet by dispase Br J Dermatol. 1983 May;108(5):555-60.
9: Geggel HS, Gipson IK. Removal of viable sheets of conjunctival epithelium with dispase II Invest Ophthalmol Vis Sci. 1985 Jan;26(1):15-22.
10: Cassiman JJ, Brugmans M, Van den Berghe H. Growth and surface properties of dispase dissociated human fibroblasts Cell Biol Int Rep. 1981 Feb;5(2):125-32.

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