Cy5 Fluorochrome

Product Description

Cy5 Fluorochrome
Catalog number: B2012779
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 1 mg
Molecular Weight or Concentration: 758.0 g/mol
Supplied as: Lyophilized Powder
Applications: molecular tool for various biochemical applications
Storage: -20°C
Keywords: 2-((1E,3E)-5-((E)-1-(5-carboxypentyl)-3,3-dimethyl-5-sulfoindolin-2-ylidene)penta-1,3-dien-1-yl)-1-ethyl-3,3-dimethyl-3H-indol-1-ium-5-sulfonate, inner salt, compd. with N,N-diethylethanamine
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Berg EA, Fishman JB. Labeling Antibodies with Cy5-Phycoerythrin Cold Spring Harb Protoc. 2019 Sep 3;2019(9).
2: Kekić T, Lietard J. Sequence-dependence of Cy3 and Cy5 dyes in 3′ terminally-labeled single-stranded DNA Sci Rep. 2022 Aug 31;12(1):14803.
3: Khare P, Dave KM, Kamte YS, Manoharan MA, O’Donnell LA, Manickam DS. Development of Lipidoid Nanoparticles for siRNA Delivery to Neural Cells AAPS J. 2021 Dec 6;24(1):8.
4: Conley NR, Biteen JS, Moerner WE. Cy3-Cy5 covalent heterodimers for single-molecule photoswitching J Phys Chem B. 2008 Sep 25;112(38):11878-80.
5: Mahmoud AM, de Jongh PAJM, Briere S, Chen M, Nowell CJ, Johnston APR, Davis TP, Haddleton DM, Kempe K. Carboxylated Cy5-Labeled Comb Polymers Passively Diffuse the Cell Membrane and Target Mitochondria ACS Appl Mater Interfaces. 2019 Aug 28;11(34):31302-31310.
6: Hagner-McWhirter Å, Laurin Y, Larsson A, Bjerneld EJ, Rönn O. Cy5 total protein normalization in Western blot analysis Anal Biochem. 2015 Oct 1;486:54-61.
7: Kim JM, Seong BL. Highly chromophoric Cy5-methionine for N-terminal fluorescent tagging of proteins in eukaryotic translation systems Sci Rep. 2017 Sep 14;7(1):11642.
8: Lam K, Tajkhorshid E. Membrane Interactions of Cy3 and Cy5 Fluorophores and Their Effects on Membrane-Protein Dynamics Biophys J. 2020 Jul 7;119(1):24-34.
9: Schmitt RR, Mahajan SD, Pliss A, Prasad PN. Small molecule based EGFR targeting of biodegradable nanoparticles containing temozolomide and Cy5 dye for greatly enhanced image-guided glioblastoma therapy Nanomedicine. 2022 Apr;41:102513.
10: Ruano-Salguero JS, Lee KH. Adsorptive-Mediated Endocytosis of Sulfo-Cy5-Labeled IgG Causes Aberrant IgG Processing by Brain Endothelial-Like Cells Mol Pharm. 2020 Nov 2;17(11):4280-4285.

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