4-Nitrophenyl β-D-Glucopyranoside Monohydrate


Catalog Number: B2012362 (100 mg)
4-Nitrophenyl β-D-Glucopyranoside Monohydrate is a high quality substrate for β-D-Glucosidase. This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

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4-Nitrophenyl β-D-Glucopyranoside Monohydrate
Catalog number: B2012362
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 100 mg
Molecular Weight or Concentration: 319.3 g/mol
Supplied as: Powder
Applications: molecular tool for various biochemical applications
Storage: -20°C
Keywords: 4-Nitrophenyl β-D-Glucopyranoside
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

1: Alhifthi A, Williams SJ. Unimolecular, Bimolecular, and Intramolecular Hydrolysis Mechanisms of 4-Nitrophenyl β-d-Glucopyranoside J Org Chem. 2021 Jul 16;86(14):9530-9539.
2: Tokutake S, Kotani K, Saito K, Yamaji N. Syntheses of 2-chloro-4-nitrophenyl beta-D-maltopentaosides with bulky modification and their application to the differential assay of human alpha-amylases Chem Pharm Bull (Tokyo). 1992 Sep;40(9):2531-6.
3: Johnson WG, Gal AE, Miranda AF, Pentchev PG. Diagnosis of adult Gaucher disease: use of a new chromogenic substrate, 2-hexadecanoylamino-4-nitrophenyl-beta-D-glucopyranoside, in cultured skin fibroblasts Clin Chim Acta. 1980 Mar 14;102(1):91-7.
4: Khan SH, Matta KL. Synthesis of 4-nitrophenyl O-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)- (1–>2)-O-(6-O-methyl-alpha-D-mannopyranosyl)-(1–>6)-beta-D- glucopyranoside and its 4′,6′-di-O-methyl analog. Potential inhibitors of N-acetylglucosaminyl-transferase V (GnT-V) Carbohydr Res. 1993 Apr 23;243(1):29-42.
5: Thomas RL, Dubey R, Abbas SA, Matta KL. Synthesis of O-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)-(1—-3)-O-beta-D- galactopyranosyl-(1—-4)-2-acetamido-2-deoxy-D-glucopyranose and two related trisaccharides containing the “lacto-N-biose II” unit Carbohydr Res. 1987 Nov 15;169:201-12.
6: Yan S, Wu G. Prediction of Michaelis-Menten constant of beta-glucosidases using nitrophenyl-beta-D-glucopyranoside as substrate Protein Pept Lett. 2011 Oct;18(10):1053-7.
7: Ohnishi T, Higashi S, Mizuma T, Awazu S. Transport mechanisms of a glycoside, p-nitrophenyl-beta-D-glucopyranoside, across rat small intestinal brush-border membranes Biochim Biophys Acta. 1998 Mar 13;1370(2):192-8.
8: Barns RJ, Clague AE. An improved procedure for diagnosis of Gaucher disease using cultured skin fibroblasts and the chromogenic substrate, 2-hexadecanoylamino-4-nitrophenyl-beta-D-glucopyranoside Clin Chim Acta. 1982 Mar 26;120(1):57-63.
9: Mayer C, Vocadlo DJ, Mah M, Rupitz K, Stoll D, Warren RA, Withers SG. Characterization of a beta-N-acetylhexosaminidase and a beta-N-acetylglucosaminidase/beta-glucosidase from Cellulomonas fimi FEBS J. 2006 Jul;273(13):2929-41.
10: Shen H, Gao XJ, Li T, Jing WH, Han BL, Jia YM, Hu N, Yan ZX, Li SL, Yan R. Ginseng polysaccharides enhanced ginsenoside Rb1 and microbial metabolites exposure through enhancing intestinal absorption and affecting gut microbial metabolism J Ethnopharmacol. 2018 Apr 24;216:47-56.

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