Standard PCR Protocol with Agarose Gel Purification

Standard PCR Protocol with Agarose Gel Purification

Standard PCR Protocol with Agarose Gel Purification

This protocol details:

  • How to perform a standard PCR reaction
  • How to extract the target PCR band from an agarose gel
  • How to digest the target PCR band
  • How to phenol extract the digested PCR band and have it ready for ligation
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Protocol is courtesy of Molecular Depot.

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