Inclusion Bodies: Preventing their Formation

Inclusion Bodies: Preventing their Formation

Inclusion bodies are often made of denatured aggregates of inactive proteins. Although, the renaturation of inclusion bodies can sometimes lead to the solubilisation and the recovery of active proteins, the process is still very empirical, uncertain and of low efficiency. Several techniques have been developed over the years to prevent the formation of inclusion bodies. These techniques include:

  • The use of weaker promoters to slowdown the rate of protein expression
  • The use of low copy number plasmids to slowdown protein expression
  • Fusing the target protein to a soluble partner such as MalE
  • The use of NativeFolder bacterial expession medium

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