Long non-coding RNA SPRY4-IT1 promotes epithelial-mesenchymal transition of cervical cancer by regulating the miR-101-3p/ZEB1 axis.
Authors of this article are:
Fan MJ, Zou YZ, He PJ, Zhang S, Sun XM, Li CZ.
A summary of the article is shown below:
BACKGROUND: Emerging evidences have indicated that long non-coding RNAs (LncRNAs) play vital roles in cancer development and progression. Previous studies have suggested that overexpression of SPRY4-IT1 predicates poor prognosis and promotes tumor progress in cervical cancer (CC). However, the underlying mechanism of SPRY4-IT1 in cervical cancer remains unknown. The aim of this study is to evaluate the function and mechanism of SPRY4-IT1 in cervical cancer.METHODS: SPRY4-IT1 was detected by quantitative PCR. Wound-healing assay and Transwell assay were performed to detect cell migration and invasion, respectively. Western blotting assays were used to analyze the protein expression of E-cadherin, N-cadherin and vimentin. Tumor xenografts experiments were performed to detect the effect of SPRY4-IT1 in vivo. Dual luciferase reporter assay was used to investigate potential molecular mechanism of SPRY4-IT1 in cervical cancer cells.RESULTS: SPRY4-IT1 was up-regulated in cervical cancer cell lines. Knockdown of SPRY4-IT1 significantly inhibited cervical cancer cells migration and invasion in vitro and in vivo Moreover, knockdown of SPRY4-IT1 significantly suppressed the epithelial-mesenchymal transition (EMT) of cervical cancer by increased E-cadherin expression and decreased the N-cadherin and vimentin expression. Mechanically, SPRY4-IT1 could directly bind to miR-101-3p and effectively act as a competing endogenous RNA (ceRNA) for miR-101-3p to regulate the expression of the target gene ZEB1.CONCLUSIONS: Our findings indicate that the SPYR4-IT1/miR-101-3p /ZEB1 axis contributes to cervical cancer migration and invasion, which may provide novel insights into the function of lncRNA-driven tumorigenesis of cervical cancer.©2019 The Author(s).
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This article is a good source of information and a good way to become familiar with topics such as: EMT; Long non-coding RNA; SPRY4-IT1; cervical Cancer; metastasis; miR-101-3p.