[Expression of costimulatory molecules B7H3 and B7H4 in T lymphoblastic lymphoma/leukemia and clinical significance].
Authors of this article are:
Zhang GZ, Li J, Bai WQ, Bu P, Wang HW, Xi YF.
A summary of the article is shown below:
in English, Chinese目的： 探讨共刺激分子B7H3、B7H4在T淋巴母细胞淋巴瘤/白血病（T-LBL/ALL）中的表达情况，及其与患者临床病理特征及预后的关系。 方法： 应用免疫组织化学法（IHC）检测2001年1月至2017年6月山西省肿瘤医院诊断的100例T-LBL/ALL（实验组）和30例淋巴结反应性增生（LH，对照组）中B7H3与B7H4蛋白表达，应用即时荧光定量聚合酶链反应（real-time RT-PCR）检测50例T-LBL/ALL和30例LH中B7H3 mRNA与B7H4 mRNA的表达。 结果： （1）男性患者79例，女性患者21例。IHC：T-LBL/ALL组中B7H3、B7H4蛋白阳性率分别为23%（23/100）与54%（54/100）。（2）real-time RT-PCR：T-LBL/ALL组中B7H3 mRNA相对表达是LH组的2.5倍。B7H4 mRNA在T-LBL/ALL组与LH组中表达量均极低。（3）单因素分析：T-LBL/ALL组中B7H3蛋白表达与出现B症状、原发淋巴结内均有相关性（P<0.05）。B7H4蛋白表达与纵隔增宽、骨髓累犯相关（P<0.05）。B7H3蛋白、B7H3 mRNA、B7H4蛋白表达和国际预后指数评分与预后相关（P<0.05），且B7H3和B7H4联合表达与T-LBL/ALL预后相关（P<0.05）。（4）多因素Cox回归分析：B7H3 mRNA表达是影响T-LBL/ALL预后的独立危险因素（P<0.05）。 结论： B7H3、B7H4表达与T-LBL/ALL临床病理参数及预后密切相关，提示B7H3、B7H4表达在T-LBL/ALL的发展中起着重要作用。.Objective: To investigate the expression of B7H3 and B7H4 in T lymphoblastic lymphoma/leukemia (T-LBL/ALL) in correlation with clinicopathological parameters and patient prognosis. Methods: Immunohistochemistry (IHC) was used to detect the expression of B7H3 and B7H4 protein in 100 cases of T-LBL/ALL(test group) and 30 cases of lymph node reactive hyperplasia (LH) (control group), diagnosed at Shanxi Cancer Hospital from January 2001 to June 2017. Real-time RT-PCR was used to detect the mRNA expression of B7H3 and B7H4 in 50 cases of T-LBL/ALL and 30 cases of LH (control group). Results: There were 79 males,21 females. Immunohistochemical results showed that the expression rates of B7H3 and B7H4 were 23%(23/100) and 54%(54/100), respectively. By real-time RT-PCR, the relative expression of B7H3 mRNA in the T-LBL/ALL group was 2.5 times of that of the LH group. The expression levels of B7H4 mRNA in T-LBL/ALL group and LH group were extremely low.Single factor analysis showed that B7H3 protein expression in T-LBL/ALL group was associated with B symptoms and primary nodal disease (P<0.05). B7H4 protein expression was associated with mediastinal broadening and bone marrow involvement (P<0.05). B7H3 protein, B7H3 mRNA, B7H4 protein expression and IPI score were associated with prognosis (P<0.05), and the combined expression of B7H3 and B7H4 was associated with T-LBL/ALL prognosis (P<0.05). Multivariate Cox regression analysis showed that overexpression of B7H3 mRNA was an independent risk factor for the prognosis of patients with T-LBL/ALL (P<0.05). Conclusion: Expression of B7H3 and B7H4 is closely corelated with clinicopathological parameters and prognosis of patients with T-LBL/ALL, suggesting that B7H3 and B7H4 expression play an important role in the development of T-LBL/ALL.
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This article is a good source of information and a good way to become familiar with topics such as: Leukemia; Lymphoma; Pathology, clinical.