Do arachidonic acid metabolites affect apoptosis in bovine endometrial cells with silenced PPAR genes?
Authors of this article are:
Szczepańska AA, Łupicka M, Korzekwa AJ.
A summary of the article is shown below:
Peroxisome proliferator-activated receptors (PPARs) are expressed in bovine uterus, and their agonists are arachidonic acid (AA) metabolites. We hypothesised that silencing of PPAR genes in bovine endometrial stromal cells (ESC) would change the intracellular signalling through PPAR and affect apoptosis after cell treatment with different AA metabolites. The study’s aims are detection of apoptosis and examining the influence of prostaglandins and leukotrienes on apoptosis occurring in physiological ESC and cells with silenced PPAR (α, δ, and γ) genes. Silencing the PPARα and PPARδ genes in cells resulted in increased DNA fragmentation and mRNA and protein expression of caspase (CASP) -3 and -8 (P < 0.05). Neither DNA fragmentation nor the mRNA and protein expression of CASP3 and -8 in cells with silenced PPARγ gene were changed compared to physiological cells (P > 0.05). Among PPARs, PPARα and PPARδ appear to inhibit apoptosis, and AA metabolites, as PPAR agonists, modify this process in bovine ESC.Copyright © 2019. Published by Elsevier Inc.
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This article is a good source of information and a good way to become familiar with topics such as: Apoptosis; Arachidonic acid metabolites; Cow; PPAR; Stromal cells; Uterus.