Quantitative Analysis of the sHLA-G Protein in Seminal plasma.

A new interesting article has been published in Am J Reprod Immunol. 2019 May 27:e13152. doi: 10.1111/aji.13152. and titled:

Quantitative Analysis of the sHLA-G Protein in Seminal plasma.

Authors of this article are:

Schallmoser A, Raab M, Karn T, Königsberger S, Schmidt E, Breitenbach-Koller H, Sänger N.

A summary of the article is shown below:

BACKGROUND: Recent studies revealed that maternal and embryonic contributions impact on HLA-G protein expression and might contribute to pregnancy success or failure, respectively. The main objective of this study was to examine the paternal levels of the immunoregulatory soluble human leukocyte antigen-G (sHLA-G) protein in seminal plasma and testicular biopsy samples during artificial reproductive technique treatment (ART) and to investigate possible correlations with other semen parameters, age and pregnancy outcome of the female partner.METHODS: Soluble HLA-G levels of 106 seminal plasma samples and 8 testicular biopsy samples were determined using a commercial sHLA-G ELISA kit.RESULTS: We observed a significant negative correlation of male age with total sHLA-G amount [P 0.023, R -0.221] and semen volume [P=0.047, R -0.193]. Testicular biopsy samples were analyzed and tested positively with sHLA-G ELISA. Levels of sHLA-G in seminal plasma samples from men with normozoospermia did not deviate significantly from those with reduced semen quality. No significant difference of sHLA-G levels in seminal plasma and pregnancy outcome of the female partner was detected. Our data showed that age of men with normozoospermia was significantly lower when the female partner conceived after ART treatment [P=0.016, Mann-Whitney U test].CONCLUSIONS: High sHLA-G levels in seminal plasma of the male partner appear not to be required for pregnancy but might contribute among other factors to the success of establishing and maintaining pregnancy through long term priming of the female uterine milieu. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.

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