[The expression of long non-coding RNA-LINC01410 in pancreatic cancer and its effect on proliferation and migration of pancreatic cancer cells].
Authors of this article are:
Cai M, Xu L, Shen L, Zhang J.
A summary of the article is shown below:
in English, Chinese目的： 探讨LINC01410在胰腺癌组织、对应的癌旁组织、人胰腺癌细胞株和正常胰腺导管上皮细胞株中的表达，分析其对胰腺癌细胞增殖和迁移的影响。 方法： 采用实时荧光定量PCR（RT-qPCR）法检测16例胰腺癌组织及对应癌旁组织的LINC01410的表达水平，检测LINC01410在胰腺癌细胞株 AsPC-1、CAPAN-1、SW1990、BxPC-3和CFPAC-1和人正常胰腺导管上皮细胞株HPDE6-C7中的表达。在表达量最高的胰腺癌 细胞株转染干扰质粒（shRNA）降低LINC01410的表达，分别采用CCK-8法、集落形成实验、Transwell小室细胞实验检测胰腺癌细胞的 活力、增殖能力和迁移能力，生物信息学预测LINC01410互补配对的miRNA及下游基因，RT-qPCR检测miRNA和下游基因mRNA的表 达，Western blot检测下游基因蛋白的表达。 结果： 胰腺癌组织中LINC01410表达水平明显高于癌旁组织[（3.46±0.32）比（0.65±0.08），P<0.01]。胰腺癌细胞株中 LINC01410表达水平显著高于人正常胰腺导管上皮细胞（P<0.05），BxPC-3细胞中LINC01410的表达水平最高 （P<0.01）。抑制LINC01410在胰腺癌细胞株BxPC-3表达后，细胞活力降低（P<0.01），细胞增殖能力明显抑制 （P<0.05），细胞迁移能力降低（P<0.05）。LINC01410可互补配对miR-497-5p，miR-497-5p可互补配对 IFITM3。抑制LINC01410表达后，miR-497-5p的表达量增加[（1.04±0.17）比 （5.79±0.43），P<0.01]，IFITM3的mRNA表达量降低[（0.39±0.05）比 （1.00±0.03），P<0.01]，IFITM3、CDK6、Cyclin D2、PCNA、Vimentin、N-cadherin蛋白的表达降低。 结论： 胰腺癌组织和细胞株中的LINC01410表达增加，下调LINC01410表达可抑制胰腺癌BxPC-3细胞的增殖和迁移，其机制可能与调节miR- 497-5p及IFITM3基因表达密切相关。.Objective: To explore the expression of long non-coding RNA-LINC01410 in the pancreatic cancer tissues, corresponding paracancerous tissues, human pancreatic cancer cell lines and normal pancreatic ductal epithelial cell line, and analyze the effect of LINC01410 on pancreatic cancer cell proliferation and migration. Methods: Real-time fluorescent quantitative PCR (RT-qPCR) was used to detect the expression level of LINC01410 in 16 cases of pancreatic cancer tissue and its adjacent tissues. RT-qPCR was performed to analyze LINC01410 expression in the pancreatic cancer cell lines AsPC-1, CAPAN-1, SW1990, BxPC-3 and CFPAC-1, and human normal pancreatic ductal epithelial cell line HPDE6-C7. Transfection of interference plasmid (shRNA) in the pancreatic cancer cell line with the highest expression level of LINC01410 were used to knock-down the expression of LINC01410. CCK-8 assay, colony formation assay, and transwell chamber assay were performed to detect the proliferation and migration of pancreatic cancer cells. The complementary paired miRNAs and downstream genes of LINC01410 were predicted by bioinformatics. The expression of miRNA and downstream genes was detected by RT-qPCR, and the protein expression of downstream genes was determined by Western blot. Results: The expression of LINC01410 in the pancreatic cancer tissues was significantly higher than that in the adjacent tissues [(3.46±0.32) vs (0.65±0.08), P<0.01]. The expression levels of LINC01410 in the pancreatic cancer cell lines were significantly higher than that in the normal human pancreatic ductal epithelial cells (P<0.05). The expression of LINC01410 was highest in BxPC-3 cells (P<0.01). After knock-down of the LINC01410 expression in the pancreatic cancer cell line BxPC-3, the cell proliferation was significantly inhibited (P<0.05), and the cell migration ability was decreased (P<0.05). LINC01410 complementarily paired with miR-497-5p, and miR-497-5p complementarily paired with IFITM3. After inhibiting the expression of LINC01410, the expression of miR-497-5p was increased [(1.04±0.17) vs (5.79±0.43), P<0.01], the mRNA expression of IFITM3 was decreased [(0.39±0.05) vs (1.00±0.03), P<0.01], and the protein expression of IFITM3, CDK6, Cyclin D2, PCNA, Vimentin, and N-cadherin was decreased. Conclusions: The expression of LINC01410 was increased in pancreatic cancer tissues and cell lines. Down-regulation of LINC01410 expression inhibits the proliferation and migration of pancreatic cancer BxPC-3 cells, and its mechanism may be closely related to regulating the miR-497-5p and IFITM3 gene expression.
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This article is a good source of information and a good way to become familiar with topics such as: Cell migration; Long non-coding RNA; Pancreatic cancer; microRNA.