miR-455-5p overexpression reduces rat lung alveolar type II cell proliferation by down-regulating STRA6.
Authors of this article are:
Zheng J, He Q, Tang H, Xia H.
A summary of the article is shown below:
miR-455-5p and retinoid signaling pathway and its membrane receptor, STRA6 are associated with lung development. Software co-predictions indicate that the miRNA upstream of the STRA6 gene is miR-455-5p. We hypothesized that miR-455-5p participates in rat lung alveolar type II cell proliferation by targeting STRA6 and designed this study to investigate the effects of miR-455-5p overexpression on rat lung alveolar type II cells. Dual luciferase reporter gene assay was utilized to confirm the relationship between miR-455-5p and STRA6. A miR-455-5p-expressing adenoviral vector was constructed and transfected into rat lung alveolar type II cells. STRA6 protein expression was detected in rat lung alveolar type II cells by Western blotting at 72 h post-transfection. Retinol concentration was detected by ELISA at 72 h post-transfection. The cell proliferation was detected by CCK8 assay at 24, 48 and 72h post-transfection. Our results showed that STRA6 is a target gene of miR-455-5p. STRA6 protein expression was significantly lower in the miR-455-5p-overexpression group than in the NC group (0.615±0.131 VS 0.958±0.246, P=0.029). Similar results were observed for retinol concentration (2.985±0.061 VS 3.949±0.118, P=0.000). Rat lung alveolar type II cell proliferation was lower in the miR-455-5p-overexpression group than in the NC group at 24, 48 and 72h post-transfection (24h: 0.280±0.184 VS 1.354±0.169 P=0.026; 48h: 0.881±0.016 VS 1.992±0.050 P=0.001; 72h: 2.105±0.148 VS 2.937±0.079 P=0.016). In summary, miR-455-5p is associated with lung development. miR-455-5p overexpression down-regulates STRA6, leading to reduced retinol concentration and rat lung alveolar type II cell proliferation. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.
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This article is a good source of information and a good way to become familiar with topics such as: STRA6; lung development; miR-455-5p; retinoid signaling pathway.