Production of recombinant proteins through sequestration in chloroplasts: a strategy based on nuclear transformation and post-translational protein…
Authors of this article are:
Muthamilselvan T, Kim JS, Cheong G, Hwang I.
A summary of the article is shown below:
Recently, plants have emerged as a lucrative alternative system for the production of recombinant proteins, as recombinant proteins produced in plants are safer and cheaper than those produced in bacteria and animal cell-based production systems. To obtain high yields in plants, recombinant proteins are produced in chloroplasts using different strategies. The first strategy is based on chloroplast transformation, followed by gene expression and translation in chloroplasts. This has proven to be a powerful approach for the production of proteins at high levels. The second approach is based on nuclear transformation, followed by post-translational import of proteins from the cytosol into chloroplasts. In the nuclear transformation approach, foreign genes are stably integrated into the nuclear genome or transiently expressed in the nucleus by non-integrating T-DNA. Although this approach also has great potential for protein production at high levels, it has not been thoroughly investigated. In this review, we focus on nuclear transformation-based protein expression and its subsequent sequestration in chloroplasts, and summarize the different strategies used for high-level production of recombinant proteins. We also discuss future directions for further improvements in protein production in chloroplasts through nuclear transformation-based gene expression.
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This article is a good source of information and a good way to become familiar with topics such as: Agrobacterium-mediated transformation; Chloroplast; Nuclear transformation of foreign genes; Plant-produced recombinant proteins; Protein import.