Tris-Glycine Running Buffer 10X (SDS-PAGE)
$150.00
Catalog Number: B2010037 (100 mL)
Tris-Glycine SDS-PAGE Running Buffer 10X. Dilute 1X with di-water for use as SDS-PAGE running buffer. Buffer generates reproducible highly resolved protein bands. Custom bulk orders are available upon request.
In stock
Description
Tris-Glycine Running Buffer 10X (SDS-PAGE)
Catalog number: | B2010037 |
Lot number: | Batch Dependent |
Expiration date: | Batch Dependent |
Volume/Weight | 100 mL |
Provided as | 10X concentrate (dilute with di-water to 1X before use) |
pH | 8.5-8.7 |
CAS Number | 288-32-4 |
Color | Colorless |
Shelf-Life | 2 years from date of manufacture |
Storage: | 4°C to 25°C |
Keywords: | SDS PAGE running buffer, running buffer, tris-glycine running buffer |
Grade | Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um. |
References | 1: Haider SR, Sharp BL, Reid HJ. A comparison of Tris-glycine and Tris-tricine buffers for the electrophoretic separation of major serum proteins. J Sep Sci. 2011 Sep;34(18):2463-7.2: Zhang Y, Wang Y, Li Y. Major cause of antibody artifact bands on non-reducing SDS-PAGE and methods for minimizing artifacts. Protein Expr Purif. 2019 Dec;164:105459.3: Hachmann JP, Amshey JW. Models of protein modification in Tris-glycine and neutral pH Bis-Tris gels during electrophoresis: effect of gel pH. Anal Biochem. 2005 Jul 15;342(2):237-45. doi: 10.1016/j.ab.2005.04.015. PMID: 15935323. 4: Litovchick L. Resolving Proteins for Immunoblotting by Gel Electrophoresis. 5: Ramos Y, Besada V, Castellanos-Serra L. Peptide fractionation by SDS-free 6: Moebius J, Denker K, Sickmann A. Ruthenium (II) tris-bathophenanthroline 7: Ladner-Keay CL, Turner RJ, Edwards RA. Fluorescent Protein Visualization 8: Jimenez MS, Luque-Alled JM, Gomez T, Castillo JR. Evaluation of agarose gel 9: Jiménez MS, Rodriguez L, Bertolin JR, Gomez MT, Castillo JR. Evaluation of 10: Marshall T, Williams KM. Analysis of snake venoms by sodium dodecyl sulfate- |
Related Products | Biochemical Buffers and Solutions |
Additional information
Weight | 48 oz |
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Dimensions | 8 × 8 × 8 in |
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