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Tris-Glycine Running Buffer 10X (SDS-PAGE)

$150.00

Catalog Number: B2010037 (100 mL)
Tris-Glycine SDS-PAGE Running Buffer 10X. Dilute 1X with di-water for use as SDS-PAGE running buffer. Buffer generates reproducible highly resolved protein bands. Custom bulk orders are available upon request.

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Description

Tris-Glycine Running Buffer 10X (SDS-PAGE)

Catalog number: B2010037
Lot number: Batch Dependent
Expiration date: Batch Dependent
Volume/Weight 100 mL
Provided as 10X concentrate (dilute with di-water to 1X before use)
pH 8.5-8.7
CAS Number 288-32-4
Color Colorless
Shelf-Life 2 years from date of manufacture
Storage: 4°C to 25°C
Keywords: SDS PAGE running buffer, running buffer, tris-glycine running buffer
Grade Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.
References 1: Haider SR, Sharp BL, Reid HJ. A comparison of Tris-glycine and Tris-tricine
buffers for the electrophoretic separation of major serum proteins. J Sep Sci.
2011 Sep;34(18):2463-7.2: Zhang Y, Wang Y, Li Y. Major cause of antibody artifact bands on non-reducing
SDS-PAGE and methods for minimizing artifacts. Protein Expr Purif. 2019
Dec;164:105459.3: Hachmann JP, Amshey JW. Models of protein modification in Tris-glycine and
neutral pH Bis-Tris gels during electrophoresis: effect of gel pH. Anal Biochem.
2005 Jul 15;342(2):237-45. doi: 10.1016/j.ab.2005.04.015. PMID: 15935323.

4: Litovchick L. Resolving Proteins for Immunoblotting by Gel Electrophoresis.
Cold Spring Harb Protoc. 2018 Oct 1;2018(10).

5: Ramos Y, Besada V, Castellanos-Serra L. Peptide fractionation by SDS-free
polyacrylamide gel electrophoresis for proteomic analysis via DF-PAGE. Methods
Mol Biol. 2012;869:197-204.

6: Moebius J, Denker K, Sickmann A. Ruthenium (II) tris-bathophenanthroline
disulfonate is well suitable for Tris-Glycine PAGE but not for Bis-Tris gels.
Proteomics. 2007 Feb;7(4):524-527.

7: Ladner-Keay CL, Turner RJ, Edwards RA. Fluorescent Protein Visualization
Immediately After Gel Electrophoresis Using an In-Gel Trichloroethanol
Photoreaction with Tryptophan. Methods Mol Biol. 2018;1853:179-190.

8: Jimenez MS, Luque-Alled JM, Gomez T, Castillo JR. Evaluation of agarose gel
electrophoresis for characterization of silver nanoparticles in industrial
products. Electrophoresis. 2016 May;37(10):1376-83.

9: Jiménez MS, Rodriguez L, Bertolin JR, Gomez MT, Castillo JR. Evaluation of
gel electrophoresis techniques and laser ablation-inductively coupled plasma-
mass spectrometry for screening analysis of Zn and Cu-binding proteins in
plankton. Anal Bioanal Chem. 2013 Jan;405(1):359-68.

10: Marshall T, Williams KM. Analysis of snake venoms by sodium dodecyl sulfate-
polyacrylamide gel electrophoresis and two-dimensional electrophoresis. Appl
Theor Electrophor. 1994;4(1):25-31.

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