Recombinant T4 DNA Ligase
$395.00
Catalog Number: B2012958 (20 KU)
Recombinant T4 DNA Ligase is a high quality recombinant enzyme that catalyzes the formation of DNA molecules from complementary single-stranded DNA. This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.
Live enquiry about this product via Text/SMS: 1-858-900-3210.
In stock
Description
Recombinant T4 DNA Ligase
Catalog number: B2012958
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 20 KU
Molecular Weight or Concentration: N/A
Supplied as: Lyophilized Powder
Applications: molecular tool for various biochemical applications
Storage: -20°C
Keywords: DNA ligase 4, EC 6.5.1.1, DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4.
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.
References:
1: Tomkinson AE, Naila T, Khattri Bhandari S. Altered DNA ligase activity in human disease Mutagenesis. 2020 Feb 13;35(1):51-60.
2: Gibson DG. Enzymatic assembly of overlapping DNA fragments Methods Enzymol. 2011;498:349-61.
3: Lieber MR. The mechanism of double-strand DNA break repair by the nonhomologous DNA end-joining pathway Annu Rev Biochem. 2010;79:181-211.
4: Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kühn R. Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells Nat Biotechnol. 2015 May;33(5):543-8.
5: Goff NJ, Brenière M, Buehl CJ, de Melo AJ, Huskova H, Ochi T, Blundell TL, Mao W, Yu K, Modesti M, Meek K. Catalytically inactive DNA ligase IV promotes DNA repair in living cells Nucleic Acids Res. 2022 Oct 28;50(19):11058-11071.
6: Williamson A, Pedersen H. Recombinant expression and purification of an ATP-dependent DNA ligase from Aliivibrio salmonicida Protein Expr Purif. 2014 May;97:29-36.
7: Kimoto M, Soh SHG, Tan HP, Okamoto I, Hirao I. Cognate base-pair selectivity of hydrophobic unnatural bases in DNA ligation by T4 DNA ligase Biopolymers. 2021 Jan;112(1):e23407.
8: Francis DB, Kozlov M, Chavez J, Chu J, Malu S, Hanna M, Cortes P. DNA Ligase IV regulates XRCC4 nuclear localization DNA Repair (Amst). 2014 Sep;21:36-42.
9: Takahashi F, Funabashi H, Mie M, Endo Y, Sawasaki T, Aizawa M, Kobatake E. Activity-based in vitro selection of T4 DNA ligase Biochem Biophys Res Commun. 2005 Oct 28;336(3):987-93.
10: Han L, Masani S, Hsieh CL, Yu K. DNA ligase I is not essential for mammalian cell viability Cell Rep. 2014 Apr 24;7(2):316-320.
Products Related to Recombinant T4 DNA Ligase can be found at Enzymes
Additional information
| Weight | 48 oz |
|---|---|
| Dimensions | 8 × 8 × 8 in |
How to Order
1- Order online using our website’s automated shopping cart and checkout system (available 24/7).
2- Order by email: info@moleculardepot.com (we accept electronic PO, available 24/7).
3- Order by Fax: 1-858-437-9884 (available 24/7).
4- Order by phone: 1-858-900-3210 (6 am – 8 pm PST).
5- Order by Text/SMS: 1-858-900-3210 (6 am – 8 pm PST).
