p-Nitrophenyl Phosphate (pNPP)

Description

p-Nitrophenyl Phosphate (pNPP)
Catalog number: B2013804
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 250 mg
Molecular Weight or Concentration: 371.1
Supplied as: Powder
Applications: molecular tool for various biochemical applications
Storage: -20°C
Keywords: p-Nitrophenyl Phosphate (pNPP)
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
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2: Qi W, Fu Y, Zhao M, He H, Tian X, Hu L, Zhang Y. Electrochemiluminescence resonance energy transfer immunoassay for alkaline phosphatase using p-nitrophenyl phosphate as substrate Anal Chim Acta. 2020 Feb 8;1097:71-77.
3: Li T, Zhong W, Jing C, Li X, Zhang T, Jiang C, Chen W. Enhanced Hydrolysis of p-Nitrophenyl Phosphate by Iron (Hydr)oxide Nanoparticles: Roles of Exposed Facets Environ Sci Technol. 2020 Jul 21;54(14):8658-8667.
4: Park SC, Smith TJ, Bisesi MS. Bioactivation of bis[p-nitrophenyl]phosphate by phosphoesterases of the earthworm, Lumbricus terrestris Drug Chem Toxicol. 1993;16(1):111-6.
5: Merkx M, Pinkse MW, Averill BA. Evidence for nonbridged coordination of p-nitrophenyl phosphate to the dinuclear Fe(III)-M(II) center in bovine spleen purple acid phosphatase during enzymatic turnover Biochemistry. 1999 Aug 3;38(31):9914-25.
6: Wang S, Tabernero L, Zhang M, Harms E, Van Etten RL, Stauffacher CV. Crystal structures of a low-molecular weight protein tyrosine phosphatase from Saccharomyces cerevisiae and its complex with the substrate p-nitrophenyl phosphate Biochemistry. 2000 Feb 29;39(8):1903-14.
7: Montalibet J, Skorey KI, Kennedy BP. Protein tyrosine phosphatase: enzymatic assays Methods. 2005 Jan;35(1):2-8.
8: Green MR, Sambrook J. Dephosphorylation of DNA Fragments with Alkaline Phosphatase Cold Spring Harb Protoc. 2020 Aug 3;2020(8):100669.
9: Takai A, Mieskes G. Inhibitory effect of okadaic acid on the p-nitrophenyl phosphate phosphatase activity of protein phosphatases Biochem J. 1991 Apr 1;275 ( Pt 1)(Pt 1):233-9.
10: Tagad CK, Sabharwal SG. Purification and characterization of acid phosphatase from Macrotyloma uiflorum seeds J Food Sci Technol. 2018 Jan;55(1):313-320.

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