p-Nitrophenyl Phosphate (pNPP)


Catalog Number: B2013804 (250 mg)
p-Nitrophenyl Phosphate (pNPP) is a high quality p-Nitrophenyl Phosphate (pNPP). This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

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p-Nitrophenyl Phosphate (pNPP)
Catalog number: B2013804
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 250 mg
Molecular Weight or Concentration: 371.1
Supplied as: Powder
Applications: molecular tool for various biochemical applications
Storage: -20°C
Keywords: p-Nitrophenyl Phosphate (pNPP)
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

1: Ansotegui IJ, Melioli G, Canonica GW, Caraballo L, Villa E, Ebisawa M, Passalacqua G, Savi E, Ebo D, G_mez RM, Luengo Snchez O, Oppenheimer JJ, Jensen-Jarolim E, Fischer DA, Haahtela T, Antila M, Bousquet JJ, Cardona V, Chiang WC, Demoly PM, DuBuske LM, Ferrer Puga M, Gerth van Wijk R, Gonzlez D_az SN, Gonzalez-Estrada A, Jares E, Kalpakliolu AF, Kase Tanno L, Kowalski ML, Ledford DK, Monge Ortega OP, Morais Almeida M, Pfaar O, Poulsen LK, Pawankar R, Renz HE, Romano AG, Rosrio Filho NA, Rosenwasser L, Snchez Borges MA, Scala E, Senna GE, Sisul JC, Tang MLK, Thong BY, Valenta R, Wood RA, Zuberbier T. IgE allergy diagnostics and other relevant tests in allergy, a World Allergy Organization position paper World Allergy Organ J. 2020 Feb 25;13(2):100080.
2: Qi W, Fu Y, Zhao M, He H, Tian X, Hu L, Zhang Y. Electrochemiluminescence resonance energy transfer immunoassay for alkaline phosphatase using p-nitrophenyl phosphate as substrate Anal Chim Acta. 2020 Feb 8;1097:71-77.
3: Li T, Zhong W, Jing C, Li X, Zhang T, Jiang C, Chen W. Enhanced Hydrolysis of p-Nitrophenyl Phosphate by Iron (Hydr)oxide Nanoparticles: Roles of Exposed Facets Environ Sci Technol. 2020 Jul 21;54(14):8658-8667.
4: Park SC, Smith TJ, Bisesi MS. Bioactivation of bis[p-nitrophenyl]phosphate by phosphoesterases of the earthworm, Lumbricus terrestris Drug Chem Toxicol. 1993;16(1):111-6.
5: Merkx M, Pinkse MW, Averill BA. Evidence for nonbridged coordination of p-nitrophenyl phosphate to the dinuclear Fe(III)-M(II) center in bovine spleen purple acid phosphatase during enzymatic turnover Biochemistry. 1999 Aug 3;38(31):9914-25.
6: Wang S, Tabernero L, Zhang M, Harms E, Van Etten RL, Stauffacher CV. Crystal structures of a low-molecular weight protein tyrosine phosphatase from Saccharomyces cerevisiae and its complex with the substrate p-nitrophenyl phosphate Biochemistry. 2000 Feb 29;39(8):1903-14.
7: Montalibet J, Skorey KI, Kennedy BP. Protein tyrosine phosphatase: enzymatic assays Methods. 2005 Jan;35(1):2-8.
8: Green MR, Sambrook J. Dephosphorylation of DNA Fragments with Alkaline Phosphatase Cold Spring Harb Protoc. 2020 Aug 3;2020(8):100669.
9: Takai A, Mieskes G. Inhibitory effect of okadaic acid on the p-nitrophenyl phosphate phosphatase activity of protein phosphatases Biochem J. 1991 Apr 1;275 ( Pt 1)(Pt 1):233-9.
10: Tagad CK, Sabharwal SG. Purification and characterization of acid phosphatase from Macrotyloma uiflorum seeds J Food Sci Technol. 2018 Jan;55(1):313-320.

Products Related to p-Nitrophenyl Phosphate (pNPP) can be found at Substrates

Additional information

Weight 48 oz
Dimensions 8 × 8 × 8 in

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