Fluorescein Biotin Conjugate

Description

Fluorescein Biotin Conjugate
Catalog number: B2011436
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 2.5 mg
Molecular Weight or Concentration: 831 g/mol
Supplied as: Lyophilized
Applications: molecular tool for various biochemical applications
Storage: -20 °C
Keywords: 5-((N-(5-(N-(6-(Biotinoyl)amino)hexanoyl)amino)pentyl)thioureidyl) fluorescein
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Haack RA, Swift KM, Ruan Q, Himmelsbach RJ, Tetin SY. The photostability of the commonly used biotin-4-fluorescein probe Anal Biochem. 2017 Aug 15;531:78-82.
2: Oberbichler E, Wiesauer M, Schlögl E, Stangl J, Faschinger F, Knör G, Gruber HJ, Hytönen VP. Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein Methods Enzymol. 2020;633:1-20.
3: Nanda JS, Lorsch JR. Labeling a protein with fluorophores using NHS ester derivitization Methods Enzymol. 2014;536:87-94.
4: Stanley M, Martin SR, Birge M, Carbain B, Streicher H. Biotin-, fluorescein- and ‘clickable’ conjugates of phospha-oseltamivir as probes for the influenza virus which utilize selective binding to the neuraminidase Org Biomol Chem. 2011 Aug 21;9(16):5625-9.
5: Trexler M, Brusatori M, Auner G. Avidin-biotin complex-based capture coating platform for universal Influenza virus immobilization and characterization PLoS One. 2021 Feb 26;16(2):e0247429.
6: Saha S, Majumdar R, Hussain A, Dighe RR, Chakravarty AR. Biotin-conjugated tumour-targeting photocytotoxic iron(III) complexes Philos Trans A Math Phys Eng Sci. 2013 Jun 17;371(1995):20120190.
7: Ebner A, Marek M, Kaiser K, Kada G, Hahn CD, Lackner B, Gruber HJ. Application of biotin-4-fluorescein in homogeneous fluorescence assays for avidin, streptavidin, and biotin or biotin derivatives Methods Mol Biol. 2008;418:73-88.
8: Mittal R, Bruchez MP. Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots Bioconjug Chem. 2011 Mar 16;22(3):362-8.
9: Waner MJ, Mascotti DP. A simple spectrophotometric streptavidin-biotin binding assay utilizing biotin-4-fluorescein J Biochem Biophys Methods. 2008 Apr 24;70(6):873-7.
10: Keppler A, Gendreizig S, Gronemeyer T, Pick H, Vogel H, Johnsson K. A general method for the covalent labeling of fusion proteins with small molecules in vivo Nat Biotechnol. 2003 Jan;21(1):86-9.

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